One of the fundamental factors causing the emergence of Iodine Deficiency Disorders (IDD) is low environmental iodine. Epidemiological studies show that IDD problem is found in areas expressed as poor areas of iodine, but without measurement data. In Kabupaten Magelang, there is a replete area, an area that has a history of IDD problems in the past, and intervention has been made, so it is hoped that the problem can be overcome. The aim of this study was to compare environmental iodine levels in replete and non-replete areas in Magelang District. The study design was cross sectional with iodine content in water samples variable of surface and ground water. The sample size is 71 from 17 surface water points and 54 ground water points. Analysis of iodine content in water samples was done with Sandell-Kolthoff method at Laboratory of Balai Litbang GAKI Magelang. The results showed that iodine content in surface water was within a fairly wide range of 0 to 22 μg/L in the replete areaand 0 to 115 μg/L in the non-replete area. The iodine content in ground water in the replete area ranges from 0 to 77μg/L, in the non-replete area between 3to 48 μg/L. There was a significant difference of iodine content between water samples from replete area with non-replete area (P <0.05). It is necessary to maintain the sustainability of sufficiency of iodine intake especially in replete area.