Aktivitas Sitotoksik pada Sel MCF-7 dari Tumbuhan Indonesia untuk Pengobatan Tradisional Kanker Payudara

Sari Haryanti • Yuli Widiyastuti
Journal article Media Penelitian dan Pengembangan Kesehatan • 2017 Indonesia

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(Bahasa Indonesia, 8 pages)

Abstract

Indonesia is rich in a variety of potential plants as research materials based on local wisdom in the discovery of new medicinal compound. The research results have been documented in the research office of plant and herbal medicine (Ristoja) 2012. This research is a further assessment of Ristoja 2012, aims to test the cytotoxic activity of 20 parts of plants that are used for traditional treatment of cancer in Indonesia. The extraction was carried out by the infuse method using water solvent then dried in 40o C oven to obtain a dry extracts. The cytotoxicity activity test was performed on MCF-7 breast cancer cell model using MTT method, and cell profile with flow cytometry method. The results of cytotoxic test showed that 19 extracts had cytotoxic activity with IC50 100-300 μg/ml. One plant showed strong activity i.e. Gerrardanthus macrorhizus Harv. ex Benth. & Hook.f. tuber with IC50 value 73.5 μg/mL. Root tuber extract did not affect the MCF-7 cell cycle profile but increased cell population in sub G1 indicating apoptosis induction, potentially developed as chemopreventive agent.

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Media Penelitian dan Pengembangan Kesehatan

Media Penelitian dan Pengembangan Kesehatan (Health Research and Development Media) is an open ac... see more