Trihexyfenidyl is a class of anticholinergic drugs used, among others, to treat the symptoms of Parkinson’s disease and to control extrapyramidal disorders due to central nervous system drugs. However, the use of high dose trihexyfenidyl may cause renal impairment. Until now, the understanding of the pathologies of renal disorders due to trihexyfenidyl is unclear. This research was conducted to look at the histologic features and changes in the renal tubules associated with the doses used. This research was an experimental study which used 36 mice (Mus musculus) and they were divided into 4 groups. Control group was given a standard feed while the treatment group consisted of three groups were given a dose of trihexyphenidyl with 0.0208mg/20grBW/day, 0.0416mg/20grBW/day and 0.0624mg/20grBW/day for 14 days. The research was done on October-November 2016 at Pharmacology, Anatomy and Histology laboratory, Faculty of Medicine Universitas Airlangga. The results showed that the average proximal tubule diameter in control goup was 24.4±2.13μm, group 1 was 21.1± 2.98μm, group 2 was 16.2± 4.1μm, and group 3 was 11.5±0.79μm. In the treatment group, the diameter of the proximal tubule decreased in proportion to the dose administered. The smallest proximal tubular lumen diameter was seen in the highest dose-treated group.