Mango is one of the commodity of tropical fruit in Indonesia which has a great opportunities for domestic market and also export. However, quality of mango still has many problems in Indonesia. One of them is the sap contaminating the skin of mango fruit. When the stem of mango fruit is broken, the sap oozes out spreading over the fruit skin causes serious skin damages and attack of disease. The aim of this study was to determine the effectiveness of washing materials and disease protecting agent on the quality of mango fruit cv. Gedong Gincu and Arumanis. Mango fruit was harvested in farmer garden in Cirebon, West Java and Probolinggo, East Java and observation was conducted in Postharvest Laboratory of Bogor Agricultural University in November 2013 to January 2014. The experiment was designed in a completely randomized design nonfactorial that consist of nine treatments: water (control) (P0T0), water (control) + fungicide (P0T1), water (control) + yeast Cryptococcus albidus with concentration 5 g/liter 5 x 104 cell/ml (P0T2), detergent 1% + water (control) (P1T0), detergent 1% + fungicide (P1T2), detergent 1% + yeast C. albidus (P1T0), detergent 1% + Ca(OH)2 0.5% + water (control) (P2T0), detergent 1% + Ca(OH)2 0.5% + fungicide (P2T1), and detergent 1% + Ca(OH)2 0.5% + yeast C. albidus (P2T1).The result showed that the treatment of detergent 1% + Ca(OH)2 0.5% + yeast C. albidus and detergent 1% + Ca(OH)2 0.5% + fungicides 0.025 was effective to removing of sap, less of sap burn injury, lenticel spoting, protecting agent of anthracnose disease and stem end rot as compared to control on mango fruit cv. Gedong Gincu and Arumanis.