Primigravida mothers are first and pregnant mothers who have not had the experience of labor, so labor is important to know the knowledge to deal with the readiness of good delivery. Preliminary survey results from 10 primigravida mother know 70% among others less readiness to face delivery. Lack of readiness can result in delayed deliveries mother came to the health service or that known by the term 3T. The research aims to determine the relationship of knowledge primigravida mothers with maternal readiness in the face of labor in working area Deket Community Health Center Lamongan District. Observational study design using analytical and cross-sectional approach. Independent variable is the knowledge of primigravida mothers about childbirth, the dependent variable is the readiness of the mother in the face of labor. The study population is the mother of three in trimester primigravidae in working area Deket Community Health Center Lamongan District. Sample as many as 28 people were taken by total sampling. Research instrument was a questionnaire. The data in the test with a contingency coefficient with significance α: 0.05. The Result contingency coefficient value 0.034 means p <α (0.034 <0.05). From the above research in getting the conclusion H0 is rejected it means there is a relationship between knowledge of primigravida mothers with maternal readiness in the face of labor. Midwives are expected to come into play as well as provide good information about labor for primigravidae women in the presence of good care Antenatal Care during pregnancy for mothers primigravidae so with the knowledge of good birth, mothers can have a good labor readiness.