Polyethylene glycol nanoparticles (NP-PEG) have good adsorption in bioactive compounds and are considered promising vehicles. Several studies have reported the importance of the amino acid for several treatments, among them glycine that has immunomodulatory, cytoprotective and anti-inflammatory effects. The objective of this work was to evaluate the efficiency of the synthesis of polyethylene glycol (PEG) nanoparticles adsorbed with glycine (NANO-PEG/GLY) on functional activity of colostrum macrophages. Human colostrum cells were obtaining from 18 clinically healthy women and used for bioassays of cell viability, phagocytosis, microbicidal activity and cytokines concentration. It was observed that the cell viability index in the presence of NANO-PEG/GLY was above 85%. Phagocytosis rates in colostrum cells treated with glycine and in the presence of EPEC, wheareas the higest microbicidal index were observed in macerophages treated with PEG-NANO-GLY. IL-1β and TNF-α levels increased in GLY and NANO-PEG/GLY groups. The levels of IL-12 and IL-17 also increased in the macrophages cultures under the NANO-PEG/GLY treatment. In the supernatant cell culture IL-8 and IFN-γ levels were similar among the treatments. The date suggest that NANO-PEG particles produced were able to adsorb the amino acid glycine, and this new bioengineered material is capable of modulating the functional activity of human colostrum macrophages and represents an alternative route for the treatment of inflammatory deseases.