The present work was designed to study the biodiversity of fungal post-harvest decay of banana, mango and safou fruits sold in local markets in the Dschang locality, Western Region of Cameroon. A total of 90 infected fruit samples were collected from different local markets, small pieces of mouldy part were inoculated on prepared plates of Potato dextrose agar (PDA), after 7 days of incubation, pure isolated fungi were identified according to the recommended references. The pathogenicity of the some most prevalent fungi isolated was evaluated on uninfected fruits. Results obtained showed some variations in isolation frequency of the fungi from each fruit. Aspergillus, Colletotrichum, Fasarium and Veticillium were the most common genera that colonized banana, mango and safou fruits with different incidences. Cercospora capsici was present on safou (50%), C. mangiferae on mango (50.9%) and C. musae on banana (51.7%). Colletotrichum gloesosporioides appeared on banana (8.62%), mango (15%) and safou (22.92%); Colletotrichum musae on banana (22%). Cercospora spp caused injuries with lesions diameters that vary depending on the type of fruit and fungal species. Proper measures should be adopted to protect fruits from fungal decay.