Cassava brown streak disease is one of the latest outbreaks of diseases threatening cassava production in Uganda. Although, previously reported in some parts of east African coast, CBSD was not a common problem in Uganda until over a decade ago. Since, its first reported outbreak in mid 2000s, CBSD has continued to spread in many cassava growing districts of Uganda. Cassava brown streak disease manifests as a syndrome characterised by leaf chlorosis, stem and root necrosis. The infected root tubers are unfit for human consumption. Therefore, the study was conducted to assess farmers' knowledge of CBSD in the selected districts in central Uganda. Semi-structure questionnaires were used to gather information from 180 respondents from the districts of Mukono, Masaka and Wakiso on the knowledge and perception of CBSD. The findings revealed that cassava was widely grown in the three districts. However, a number of constraints including pests and diseases were reported to be affecting cassava growing. Of the diseases, CBSD was ranked as the most widespread and devastating. In fact, 75% of the respondents had good knowledge of CBSD and perceive it as responsible for the declining cassava production in the districts. The most common symptoms associated with CBSD leaf chlorosis, rotting and necrosis of the root tubers. Both the old and newly introduced cassava varieties were susceptible to CBSD. Accordingly, CBSD was thought of as responsible for food insecurity, livelihoods and the loss of cassava biodiversity among others.