In this article presents experimental data, the basic composition of the venom of Macrovipera lebetina obtusa, captured from different regions of Azerbaijan, differing in degree of contamination by industrial emissions was studied. γ – radiospectrometric studies showed that the samples of venom also contain radionuclides as Ra228, Ra226, K40 and 137 Cs. It was established that the radiation dose (up to dose 1.35 kGy) for 3 minutes did not cause structural changes in the samples venom of vipera, but rather contribute to the stabilization of both toxicity and pharmacological activity while increasing the shelf life of aqueous solutions of vipera venom. At high doses (2.7, 4.05 and 5.4 kGy) γ-irradiation for 3 minutes there was a gradual decrease in toxicity (pharmacological activity of enzymes) of snake venom. We can assume that these data can be used in the identification of zootoxins and their metabolites, and these criteria can serve as a theoretical basis for the development of effective methods for diagnosis of poisoning zootoxins.