Among the diseases that can reduce soybean production is rust disease caused by the fungus Phakopsorapachyrhizi. The aim is to evaluate the resistance of soybean genotypes to rust disease and to study there interactions between agronomic traits. The study was conducted at field and screen house in the University of Agriculture Makurdibetween julyto november2016. A total of 10 soybean genotypes were evaluated for resistance to rust disease. After inoculation in the laboratory, three lines TGX-1835-10E, TGX-1987-10F and TGX1945-4F showed a consistent moderate resistance to Phakopsorapachyrhizi. These soybeanGenotypeowered earlier and had the highest seed yield per plant (799.51kg/ha, 766.75K g/ha and 742.63 respectively). In contrast, the lines TGX-1949-10F and TGX-1485-1D which is the control , flowered at about 43 days after planting, had seed yield per plant of (404.30 and 254.23kg/ha, respectively),these lines had significantly lower yield and susceptible to rust. In the field, four lines had seed weight per plant significantly heavier than TGX-1949-10F and TGX-1485-1D, namely TGX-1835-10E, TGX-1987-10F, TGX-1904-6F and TGX-1945-4E and using a polygon view, the best performing lines were visualized as TGX-1987-10F was best in Environments one and TGX-1835-10E in two.Base on average environment coordination (AEC) procedure, TGX-1945-1F and TGX-1945-4E had yields above the grand means and stable while TGX-1945-4F and TGX-1935-3F were identified with high but unstable yield, the soybean lines with heavier seed weight per plant should potentially serve as genetic material to develop high yielding soybean varieties and resistant to rust disease.