Resistance to antibiotics is a serious matter of concern for Public and threats to the successful treatment of miscrobial disease. The prevalence of some enterobacteriaceae such as Escherichia coli and Salmonella spp resistant to antimicrobial agents is increasing. This study is intended to determine susceptibility and resistance pattern of pathogenic isolates from the rectal swabs of chicken to 8 antibiotics. Hundred (100) rectal swabs of chickens were collected randomly from three different farms; a farm at Montan, Awotan, Apete and Apata all in Ibadan Metropolis of Oyo State. Isolation and identification of organisms were done using standard bacteriological techniques. Antimicrobial susceptibility test was performed following standard protocol. A total of 93 bacterial isolates were obtained from the hundred (100) rectal swaps of chicken. The isolates Escherichia Coli, Staphylococcus aureus and Salmonella spp. and their percentage of prevalence are 54, 43 and 3. Antibiotic susceptibility tests carried out on the isolates showed that most of the isolates were resistant to ofloxacin, cloxacillin and Augumentin, while almost all the isolates in this study are sensitive to gentamicin. Erythromycin did not have any effect on any of the bacterial isolates. Bacterial isolates obtained in the study area were multi drug resistant and this suggests that the chickens are important reservoir of antimicrobial resistant organism which is a major public health concern.