Optimasi Amobilisasi Urease Dari Schizzosaccharomyces Pombe Menggunakan Matrik Kitosan-polietilen Glikol

Hastini Artha Dewi • Sasangka Prasetyawan • Chanif Mahdi
Journal article Jurnal Ilmu Kimia Universitas Brawijaya • 2013

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(Bahasa Indonesia, 7 pages)

Abstract

Schizosaccharomyces pombe is yeast that production urease and extraceluller enzyme. Urease can hydrolyze urea to be ammonia and carbondioxide. Free urease is unable to be reused, therefore urease is needed to immobilized in certain matrix. This research studies to determine optimum condition from chitosan concentration and enzyme concentration. Urease was immobilized by entrapment method using matrix chitosan-polyethylene glycol with make a variation to chitosan concentration (1.5; 2; 2.5; 3; 3.5) % (w/v) and make a variation to urease concentration (0.840; 1.680; 2.520; 3.360; 4.200) mg/mL. Enzyme activity was defined as the amount of mikromol ammonia per minute, and resulted ammonia was analyzed by Nessler method. The results showed that the optimum condition of immobilization uerase is achieved on chitosan concentration 3% (w/v) and urease concentration of 2.52 mg/mL and urease yielding in 2.36 mg/gram matrix chitosan-polyethylene glycol within the activity of 0.53 Units.

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Jurnal Ilmu Kimia Universitas Brawijaya

Jurnal Ilmu Kimia memuat publikasi hasil karya mahasiswa S1 Kimia yang berisi ide, gagasan maupun... see more