Amobilisasi Pektinase Hasil Isolasi dari Aspergillus Niger Menggunakan Matriks Karagenan

Elvanila Tanjung • Anna Roosdiana • Diah Mardiana
Journal article Jurnal Ilmu Kimia Universitas Brawijaya • 2013 Niger

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(Bahasa Indonesia, 7 pages)

Abstract

Pectinase isolated from Aspergillus Niger was immobilized by entrapping method in a matrix of carrageenan. The aim of this research was to determine the optimum concentration of carrageenan and enzyme. Carrageenan concentration varied (0.017; 0.033; 0.050; 0.067; 0.083) g/mL, while the concentration of enzyme varied (0.632; 1.263; 1.895; 2.526; 3.158) mg/mL. Protein content was determined by Biuret reagent and enzyme activity was determined from the number of reduced sugar as a result of hydrolyzed pectin per minute. The results showed that optimum concentration of carrageenan was 0.050 g/mL and the optimum enzyme concentration was 1.263 mg/mL with the amount of entrapping enzyme was 3.048 mg/g carrageenan and maximum activity of 348.5 units.

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Jurnal Ilmu Kimia Universitas Brawijaya

Jurnal Ilmu Kimia memuat publikasi hasil karya mahasiswa S1 Kimia yang berisi ide, gagasan maupun... see more