Introduction:Wound is a very common thing that can be happened to all people around the world, starts from children to adults. We can treat wounds by using modern medicine and herbs medicine. One of the most commonly used herbs in the community is garlic (Allium sativum L.).Objectives: The purpose of this research is to investigate the effect juice of garlic (Allium sativum L.) in accelerating the healing wound process.Methods: In this study, we use a comparative true experimental design method with 25 mice with average be a five groups of mice were made cut along 8 mm and treated with juice of garlic 2.5%, juice of garlic 5%, juice of garlic 10%, distilled water, 1% chloramphenicol. We observed the healing wound process until the both wound edges interlocked. Data analysis is using one-way ANOVA which is continued by Tukey HSD, α = 0,05. Results: The mean wound healing time in days with juice of garlic 2.5% (5), juice of garlic 5% (5), juice of garlic10% (5.6) show a highly significant difference compared with the control negative group with p=0,000, p=0,000, p=0,000.Conclusion: Juice of garlic (Allium sativum L.) has effect in accelerating healing wound process.