The purpose of this research to evaluate the response of growth and yield of mustard plants invarious growing media composition. This research used a randomized block design (RAK) factorial design consisting of two factors. The first factor is the concentration of liquid organic fertilizer (C) which consists of three levels ie : C1 (6 ml/l of water), C2 (8 ml/l of water) and C3 (10 ml/l of water) The second factor is variety of growing media composition (M) which consists of three levels ie : M1 (a mixture of sand + husk 1:2), M2 (sand + husk 2:1), and M3 (sand + husk 1:1). So there are 9 combinations of treatment and repeated three times so that there are 27 experimental units. The results showed the concentration of liquid organic fertilizer significant effect on the components of plant height, number of leaves, leaf area, plant fresh weight, dry weight of the plant. The concentration of liquid organic fertilizer 10 ml/l of water provide higher yields than the concentration of 8 ml/l of waterand 6 ml/l of water. The treatment of a variety of growing media composition significant effect on the components of plant height, number of leaves, and plant fresh weight. Planting medium sand + husk 1:1 gave higher yields compared to planting medium sand + husk 1:2 and 2:1.