Increased of transportation needs also increase the fuel consumption so causing scarcity. In the last 10 years, Indonesia has decreased oil and gas production therefore it needs alternative energy such as bioethanol. Community activities often leaving waste stale rice containing carbohydrates and can be converted into alcohol. This research aims to knowing comparison of water volume, yeast weight, fermentation time to produce optimal bioethanol content and testing of bioethanol characteristic.This research uses experimental method with stale rice weight 250 gram with stale rice 250 grams, water volume (750, 1000, 1250, 1500 mililiters), yeast (5, 7, 9, 11 grams) and time fermentations (3, 4, 5, 6 days) to obtained a maximum bioethanol content. Bioethanol production is processed by the stage of saccharification, fermentation with saccharomyces cerevisiae and distillation at temperature of 78 ºC. After content of bioethanol obtained at >95%, conducted characteristic testing according to the decision of the new energy director general, renewable and energy conservation number 722K/10/DJE/2013. Test data results are presented in the form of tables and charts with distributive explanations. The research results showed that the best comparison of water is 5000 mililiters, 45 grams yeast and 5 days fermentation time with 29% bioethanol content. Furthermore multilevel distillation to reach levels of 96,5% with the addition of 25 grams silica gel and limestone 30 grams at the end of the condenser as absorbent moisture content. The result examination of bioethanol characteristic of stale rice waste that is bioethanol content 96,5%, methanol 0,00103%-v, water content 0,7726%-v, copper content 0,0067 mg/kg, acidity as acetic acid 2 mg/L, brightness, no sediment, 0 mg/L chloride ion content, 50 mg/L sulfur content, gum washed content 1.2 mg/100ml, heating value 5724 kcal / kg, flash point 30°C, pour point -50°C, density 0,808 gr/cm3, viscosity 3,5 cPs and pH 7,55. There are several characteristics that exceed the standard specified by the Director General of New Energy, Renewable Energy and Conservation No. 722K / 10 / DJE / 2013 that is the sulfur content and water content which is suspected because the distillation apparatus contains various elements of metal alloy and raw material which already contains high water content that is 57%. The econometric calculation of bioethanol from stale rice waste is more expensive than the bioethanol in the market because the process of manufacture is still small scale. Keywords: Bioethanol, saccharomyces cerevisiae, saccharification, fermentation, distillation.