Pemilihan Tetua Persilangan Pada Kubis (Brassica Oleracea Var. Capitata) Melalui Analisis Keragaman Genetik [Parental Line Selection in Cabbage (Brassica Oleracea Var. Capitata) Through Genetic Diversity Analysis]

Nur Kholilatul Izzah • Nfn Reflinur
Journal article Jurnal Hortikultura • June 2018 South Korea


Cabbage (Brassica oleracea var. capitata) is one of vegetable that has high economic value. The availability of high-yielding varieties that are resistant to some diseases, particularly black rot and clubroot disease is needed in order to increase cabbage yield per year. The method which can be applied to assemble new varieties is through crossbreeding program. The objective of this research was to select the ideal combination of parental lines in cabbage through analysis of genetic diversity by using SSR markers (Simple Sequence Repeats). The research was conducted from February to May 2013 in the laboratory of Crop Functional Genomics and Biotechnology, Seoul National University, South Korea using 16 cabbage genotypes obtained from Joeun seed company, South Korea. The genetic diversity of 16 cabbage genotypes were analyzed using 35 polymorphic SSR markers, and then used to determine the genetic diversity based on UPGMA method. Meanwhile, genetic distance value among cabbage genotypes obtained by the formula of 1-value genetic similarity. The results of genetic diversity analysis divided 16 cabbage genotypes into two main heterotic groups at genetic similarity value of 65.2%. Based on the results of genetic diversity analysis and genetic distance value, we selected four combinations of ideal parental lines, namely genotype IMO-03 vs IMO-08 (genetic distance value of 43%) and IMO-03 vs IMO-10 (genetic distance value of 39%) for black rot disease resistance character, as well as genotype IMO-18 vs IMO-10 and IMO-17 vs IMO-10 with genetic distance value of 45% and 44%, respectively for club root disease resistance character. These four parental lines combination were selected as ideal parental combinations due to they were located on a different heterotic groups and has high genetic distance value, which is expected to increase the chances of heterosis in their progeny.




Jurnal Hortikultura

Jurnal Hortikultura (J.Hort) memuat artikel primer yang bersumber dari hasil penelitian hortikul... see more