Multiplikasi Tunas dan Induksi Perakaran pada Perbanyakan Rhododendron Radians J.J.Sm (Ericaceae) secara In Vitro [Shoot Multiplication And Root Induction On In Vitro Propagation Of Rhododendron Radians J.J.Sm (Ericaceae)]

Tri Warseno • Siswoyo Putri, Dyan Meiningsasi
Journal article Jurnal Hortikultura • June 2018 Indonesia

Abstract

Rhododendron radians is an ornamental plant that found only in Sulawesi (Central and North). Commercialization of this species requires mass propagated plant materials. This study aimed to determine the proper technique for R. radians micropropagation in vitro. This study used two stages of treatment i.e (1) treatment of IAA on shoot proliferation R. radians arranged in completely randomized design with one factor (eight medium combination), the concentration of plant growth regulators IAA which consisted of two levels: 0.1 mg/l, and 1 mg/l and concentration of growth regulators 2iP consisting of four levels (0,6, 7, and 8 mg / l); (2) IBA treatment on shoot growth and rooting using five variations of the medium (M1 = WPM (control), M2 = WPM + 0.25 mg/l IBA, M3 = WPM + 0.5 mg/l IBA, M4 = WPM + 1 mg/l IBA; M5 = WPM + 5 mg/l IBA). The results showed that the WPM added with 1 mg/l IAA and 7 mg/l 2iP was the best medium for shoot induction initiated from seed culture, with the average number of shoots 15. 80 ± 3.45 cm and an average shoot height of 2.36 ± 0.25 cm. While the best medium for root induction was M4 (WPM + 1 mg / l IBA), with a percentage of 63.33% rooted explants and the average root length of 4.7 mm. The results of this study can be used as a starting point to conduct mass propagation R. radians.

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Jurnal Hortikultura

Jurnal Hortikultura (J.Hort) memuat artikel primer yang bersumber dari hasil penelitian hortikul... see more