Hospital as a patient care and healing , it turns susceptible to infectious diseases , as well as occurring in RSPI Prof. Dr Sulianti Saroso . Therefore, it is necessary to study the number and types of bacteria in the environment RSPI - SS . This study was an observational study with cross selection. Types of germs that will be examined are the germs found in the environment and can move through hand contact and can transmit the disease to humans , ie fungi and bacteria . Environmental classification in this research is the area of public environment , room service and medical support space in RSPI-SS with the type of sample , ie visitor chairs , elevator buttons , attendance machine , ATM buttons , toilet faucets , doorknobs , counters , computer keyboards , up the phone , walls , tables nurse station , stethoscope , flooring , inhalation , suction , bed , inspiration ventilators , ventilator tape , push chairs , trolleys action , the patient table , tanometer , handles fleet , endoscopy , food trolley , linens trolley, and tables kitchen . The results of this study are composed of 302 samples from the surface of the specimen and the hospital environment tool found 129 samples ( 42.715 % ) were positive for bacteria . Mushroom is a type of bacteria that are most numerous in the environment RSPI Prof. Dr Sulianti Saroso ( 98 450 % ) . The floor is the most sample types discovery of germs 23 samples ( 17.8 % ) . Then the computer keyboard 18 samples ( 14.0 % ) and the bed 17 ( 13.2 % ).