Multi-wall carbon nanotubes (MWCNTs) are widely used in various fields due to their superior properties. Due to its wide-ranging research and application, the biosafety of MWCNTs should be carefully considered. In the study, we reported that the toxicity of MWCNTs and the influence on the reproductive ability to strawberry. The growth status and reproductive ability of strawberry were monitored after 60 days of soil culture in the presence of MWCNTs. MWCNTs showed adverse effects on the photosynthesis of strawberry leaves and the reproductive capacity of strawberry has been suppressed. Our results collectively suggested that the biosafety of MWCNTs should be highly concerned.