Substrates are materials, other than soils in situ, in which plants are grown, and it is often used synonymously with rooting medium. An experiment was conducted at the Crop Type Museum of the Department of Crop, Soil, and Pest Management, the Federal University of Technology, Akure, to determine the rooting development of Sansevieria trifasciata as influenced by different propagation substrates. Results showed that the performance of the Sansevieria trifasciata planted using sand as substrate enhanced growth and root development of Sansevieria trifasciata compare to other substrates used (topsoil, sawdust, and rice hull). The treatment combination of all the substrates Topsoil + Sawdust + Rice hull + Sand performed better than other treatment combinations and sole. This study provided the empirical evidence that substrate combination influenced root development of Sanseveria spp.