Plant-parasitic nematode cause economic loss to crops throughout the world. Biofumigation is the environmental friendly control option for the suppression of plant-parasitic as well as other pathogenic soil microbes. Glucosinolates are the main active compound present in some plants which are responsible for biofumigation process. To increase the efficiency of biofumigation selection of varieties containing more glucosinolates is highly desirable. Plant growth stage, soil temperature, soil texture, moisture, soil depth and soil microbes play important role in efficient biofumigation.