Many scientific studies confirmed that plants have an important ecological role maintaining the productivity of the environment and regulating the global climate. In order to valorize the wild phytogenetic resources for the efficient in situconservation and sustainable use in Sudano-zambezian region, a study was carried out in Sudanian domainprovidingquantitative informations on the community structure and natural regeneration status of woody plant species.The study site is the eastern part of National Park of Sena Oura in Chad. Adults plants (trees and shrubs) were systématically collected in 10 linear transects (20 m x 1000 m). Juvenile plants (saplings and seedlings) weresystematically collected within 40 plots (20 m × 20 m). These plots were randomly stablished in the transects, at a rate of four plots per transect.In total, 84 adults plants species grouped in 58 genera and 29 families and 66 juvenile plants species grouped in 45 genera and 27 families were inventoried. Bell andreverse J-shaped patterns of selected woody species were identified.The stand regeneration status wasgood.The stand regeneration rate were SRR = 52.29% and Hymenocardia acida (SIR = 17.95%), Combretum collinum (SIR = 14.12%), Annona Senegalensis (SIR = 6.67%) and Isoberlinia doka (SIR = 6.22%) had the most important specific index of regeneration. The specific structures showed that the structure of the total stand is the result of the dynamics of all species and their interactions.The global stand regeneration status was good.The obtained results provided quantitative informations on the community structure and natural regeneration status of woody plant species for the efficient conservation and sustainable use.