This research aimed to determine the best inclusion level of crude glycerol in the diet of the Amazon catfish (Pintado), through zootechnical performance, body composition, metabolic profile and histopathology. The experiment was conducted at the Laboratory of Morphophysiology and Biochemistry of Neotropical Fishes of the Federal University of Tocantins. There was used 150 juvenilles of pintado, these with initial weight of 6,83 ± 1,11 (g) and 10,06 ± 0,57 (cm) lenght in a completely randomised design, with 3 replications (10 animals in each one). They were fed with five diets containing increasing levels of glycerol (0 g kg-1, 50 g kg-1, 75 g kg-1, 100 g kg-1, and 125 g kg-1) during 90 days (30 days of adaption and 60 experimental days). The indexes were evaluated and they did not present statistical difference between each other, except for the specific growth rate, which showed a moderate linear behavior and muscular glycogen that at the level of 125 g kg-1 presented a lower concentration compared with the control diet (0 g kg-1). Regarding histology, the crude glycerin did not cause significant hepatic and renal changes in the referred specie, since the alterations found in the two tissues were considered lesions that did not compromise the functioning of the organ or that are reversible. Finally, it was indicated that the juveniles of Amazon Pintado are able to metabolize the crude glycerin up to 100 g kg-1level.