Culture filtrate of four rhizospheric fungi and four biocontrol agents were studied in vitro for their efficacy against Meloidogyne incognita race 2. The per cent mortality and egg hatching inhibition was proportional to the concentration of culture filtrate and the duration of exposure period. Culture filtrates of Trichoderma viride, Trichoderma harzianum, Trichoderma sp., Fusarium sp., Penicillium sp. and Aspergillus sp. significantly induced inhibition of egg hatching and mortality of Meloidogyne incognita race 2.The highest percentage of inhibition of egg hatching and juvenile mortality was recorded in Trichoderma harzianum followed by Trichoderma viride and Trichoderma sp.