Feed quality shown from level of digestibility can affect fish growth.Some of omnivorous fish have complete digestive organs as a place to live abiotic and biotic ecosystems in the form of living microflora. Growth performance can be improved through the addition of exogenous microflora as feed supplements to help produce simpler components of food substances (amino acids, fatty acids, simple sugars, organic vitamins and minerals). The microflora tested consisted of bacteria Bacillus sp. and fungi (Aspergillus Niger and Saccharomycescereviseae) with optimization of its prebiotic bioprocess conditions (bioprocess temperature, inoculum dose, and bioprocess time). Furthermore, to see the quality and value of benefits of feed supplement products, measurements were taken of their digestibility.The experiment was carried out experimentally in a laboratory in two stages. The first stage, using a nested design (3×3) which was repeated three times. The second stage used a completely randomized design, consisting of six ration treatments and repeated four times. The variables observed in the first stage: nutrient content (crude protein, crude fibre, extract ether, calcium and phosphorus) of prebioticsBAS; second stage: digestibility of dry matter and crude protein.The data were subjected to analysis of variance, and the differences between treatments were tested by Duncan's multiple range test.Conclussion: The following results were obtained the best bioprocess conditions for making PrebioticsBAS from Bacillus sp. was a dose of 2% with temperature of 45oC, and fermentation time 2 days, while Aspergillus Niger 2% at a temperature of 35oCalong 2 days, and Saccharomyces cereviseae 2% with a temperature of 35oC, and fermentation time 2 days. The use of a mixture of three types of microbial each a much 1.5% in the ration, resulting in the best digestibility value in fish. The dry matter and crude protein digestibility valueof PrebioticsBAS were respectively 76.07%, and 75.28%.