Application of fish recombinant growth hormone (frGH) has been known as one of the applicable method to improve growth performance of culture fish.The purpose of this study was to evaluate the effects of recombinant growth hormone (rGH) supplementation in commercial diet on growth, feed utilization and flesh quality in striped catfish grow out. The rGH was mixed with chicken egg yolk and sprayed on commercial feed with different protein levels (32, 28 and 23%). As control the diets were sprayed with chicken egg yolk without rGH. Striped catfish (body weight: 110,66 ± 1,32 g ind-1) were fed on rGH-supplemented diets two times a week ( first and third months), and the rest was on diet without rGH supplementation. Fish were reared for 120 days in 18 hapa (2×1×1,5 m3) with initial density of 20 fish. The result showed that the highest weight gain, specific growth rate (SGR), and lowest feed conversion ratio (FCR) were obtained in fish fed on 32% protein content of rGH-supplemented diet. Furthermore, there was no significant difference in weight gain, SGR and FCR in rGH treatment group with 28% protein content of rGH supplemented diet and non-rGH control group receiving 32% protein diet (P>0.05). Similar moisture content of meat, protein content of meat, belly fat and edible portion were observed in rGH-supplemented diet and their control. Except in treatment 23% protein content rGH supplemented diet has lower lipid content in fish body and meat were found (P<0.05). It can be concluded that the highest SGR is obtained when fish fed 32% with protein feed combined with rElGH. Enrichment with rElGH depleted the fat content in meat of fish feed all levels of protein which was the lowest fat found in 23% protein feed.