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Published 10 May 2016

Abstract

Total phenolic test on the results of partial fractionation of the extracted layers of Sea Bamboo (Isis hippuris) showed ethyl acetate fraction as much as 8.49±0.15 mg GAE/g followed by the ethanol fraction at 5.26±0.13 mg GAE/g and n-hexane fraction at 3.24±0.16 mg GAE/g. Meanwhile, the result of total flavonoids for ethanol and n-hexane fraction was 6.67%, whilst for ethyl acetate fraction was 5.13%. The test results on antioxidant activities using DPPH showed ethanol fraction as much as 1795.08 ppm, n-hexane fraction 1888.45 ppm and ethyl acetate fraction 2014.54 ppm. The results of FTIR chromatogram showed that there were 9 peaks of ethanol fraction, 10 peaks of ethyl acetate fraction and 12 peaks of n-hexane fraction. GC-MS data analysis on the outer layers of Sea Bamboo (Isis hippuris) revealed 53 compounds of ethanol fraction, 40 compounds of ethyl acetate fraction, and 50 compounds of n-hexane fraction. The most dominant compounds were 2-butoxyethanol (43.68%) of ethanol fraction, Methyl-D-mannopyranoside (20.41%) of n-hexane fraction, and 2-Myristynoyl-glycinamide (19.51%) of ethyl acetate fraction. Keywords: Sea bamboo, phenols, flavonoids, Isis hippuris, antioxidant, DPPH, GC-MS, FTIR

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