Aplikasi Ribavirin Pada Shoot Tip Bawang Merah Untuk Eliminasi Virus OYDV (Application of Ribavirin on Shoot Tip of Shallot for OYDV Eradication)

Nfn Aqlima • Bambang Sapto Purwoko • Sri Hendrastuti Hidayat • Diny Dinarti
Journal article Jurnal Hortikultura • December 2018

Abstract

Chemotherapy is an apllication of chemistry compound (ribavirin) that has antiviral activity to inhibit virus multiplication in plant tissues. The objective of the experiment was to evaluate the effect of ribavirin concentrations on shoot tip growth of shallot for OYDV eradication. The experiment was cunducted at Tissue Culture Laboratory 3, Agronomy Department and Plant Virology Laboratory, Plant Protection Department of IPB from October 2015 until June 2016. The experiment was arranged in a completely rendomized block design with two factors and four replications. Each experimental unit consisted of four bottles with one explant in it. The first factor was ribavirin concentrations, i.e. 0, 5, 10, 15, and 20 mg/L. Second factor was shoot tip sizes, i.e. 1.1–2.0 mm and 2.1–3.0 mm. The experiment showed that increasing ribavirin concentrations suppressed the shoot length of cv. Bima Brebes, and it also inhibited the time of leaf to emerge, shoot length, and leaf number of cv. Tiron. Increasing shoot tip size (2.1–3.0 mm) influenced percentage of explant growth and speed the time of leaf emergance of cv. Bima Brebes and Tiron. Ribavirin concentrations used in this treatment did not eradicate OYDV in both shoot tipe sizes of the two cultivars.

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Jurnal Hortikultura

Jurnal Hortikultura (J.Hort) memuat artikel primer yang bersumber dari hasil penelitian hortikul... see more