The study aimed to know the effect of different salinity on shrimp of protein content and protein profiles. The animal in this study was Macrobranchium sintangense shrimp with average length ± 50 mm which has acclimation for three days. The shrimp was treated at different salinity, the salinity 0 0/00, 5 0/00, 100/00,15 0/00 and 20 0/00 for seven days. Protein content and protein profiles by taking shrimp gill. Shrimp gill test showed that has a different protein content, protein profiles due to treatment of different salinity. The result was conclude that salinity 0 0/00, 5 0/00, 100/00,15 0/00 and 20 0/00 affect protein content and protein profiles of M. sintangense.