The existence of Diadema setosum found pretty much almost every Ambon Island waters but has not been used optimally. This study aims to determine the physical-chemical characteristics of some waters Diadema setosum Ambon Island. The study was conducted in June 2016 in the village of Poka Martafons coastal waters, beaches and beach Sopapei Suli Waai Waai village. The method used in this research is survey method, observation and sampling methods. The parameters observed in this study is the physical parameters of water chemistry, yield, morphometric, moisture, protein, fat, ash, carbohydrates from setosum Diadema urchins gonads. The data obtained are presented in the form of figures and tables. The results showed the diameter of the shell and the body weight of the Diadema setosum urchins coastal waters Martafons ranged from 36.5 to 61.7 mm and 23.6 -100.8 g; Sopapei coast from 34.8 to 79.8 mm and 23.8 to 107 grams; Waai coast ranged from 33 to 64.5 mm and 13.9 to 91.1 g. The average weight of the Diadema setosum urchins from Ambon Island waters ranges from 30-50 grams with a diameter of 40-50 mm shell. The growth pattern of the urchins Diadema setosum third alometrik waters are negative. The percentage yield of gonadal smaller than the shell and innards. Gonadal yield of 1.5 to 5%, while the shell reaches more than 85%. The average color of the gonads diadema setosum of coastal waters Martafons, sopapei and Waai is yellow to pale yellow compact textured, gonad weight below 10%. The water content of the gonads (73.76 to 84.13)%, ash (0.20 to 2.12)%, fat (3.47 to 5.81)%, protein (5.40 to 17.69)%, carbohydrates (2.11 to 7.50)%. In general, the physical-chemical characteristics of the sea urchin better Sopapei coastal waters of the coast and beaches Martafons Waai.