The aim of the research was to study the chemical composition and effectiveness of the seed oil of Spindle tree (Euonymus europaea L.) on the non-allergic contact dermatitis model.The oil was obtained by the extraction by petroleum ether from the seeds. The analysis of fatty acids and determination of their quantitative content was carried out using gas chromatography. Determination of carotenoids and tocopherols content in fatty oils was carried out after chromatography on a silica gel column. Investigation of anti-inflammatory action of the Spindle tree seed oil on white rats was carried. Determination of biochemical parameters of blood plasma was performed on the semi-automatic biochemical analyzer BS3000M (Poland).The yield of oil was 20–28 % of the weight of the seeds. Nine fatty acids were identified in the oil by the gas chromatography, among which 4 are unsaturated (oleic, palmitoleic, linoleic and linolenic), which together make up 87.79 % of all fatty acids from this oil. Spindle tree oil contains 26±5 mg – % carotenoids and 40±5 mg – % tocopherols, which were separated by chromatography on a silica gel column. There were no symptoms of intoxication with the introduction of Spindle tree oil in the stomach of rats and it can be classified in the 4 the grade of danger in accordance with GOST 12.1.005-88.The anti-inflammatory activity of Spindle tree oil in comparison with the oil of Sea buckthorn on the model of non-allergic contact dermatitis was weaker, but sufficient to recommend it for the treatment of skin diseases in ointments.