Background: Neck pain is a widespread problem among motorcyclists, which is often neglected. There is limited research on the motorcycle's ergonomics, particularly in the context of the interaction between the riders and motorcycle. Motorcycle helmets have proven to increase the weight on the neck, thus causing more burdens which can lead to neck pain. Methodology: Case-Control study design was opted to measure the relative odds of neck pain in relation to the helmet use as an exposure. A total of 260 (mean age of 22.58 ± S.D. 1.95 years) undergraduate students were selected using purposive sampling. The case to control ratio was 1:4 (54 Cases and 206 Controls) where cases were defined as the motorcyclists having neck pain with a riding experience of more than one year. The neck pain and disability scale were obtained using a self-administered questionnaire. Chi-square and binary logistic regression were used to calculate the significant relationship and odds of neck pain amongst motorcyclists with and without helmet use. Results: The study results showed that out of 260 motorcyclists, 190 (73.1%) were helmet users, and 54 (20.8%) had neck pain, 70 (27.9%) helmet users had a neck pain prevalence of 11 (4.2%). The relative odd to have neck pain was 2.13 times more amongst the motorcyclists using the helmet as compared to that of non-helmet users. The logistic regression results showed significant results (P < 0.05) with regards to the BMI, helmet weight and duration of helmet use but did not show a significant relation with average motorcycle use per day unless it exceeded 70 kilometres. Conclusion: Use of helmet can be a potential cause of neck pain amongst motorcyclist users but the odds to have neck pain enhance with the increase in motorcycle use per day. The protective benefits are multi-fold for helmet use which outreaches the negative impact, including neck pain amongst motorcyclists.