Tuberculosis is a direct and chronic infectious disease that is transmitted by tuberculosis sufferers, thus tuberculosis sufferers must be treated immediately until healed. Medan City, located in Indonesia, is the largest area of TB sufferers compared to other areas in North Sumatra, Indonesia. In this study, we use the Multiple Linear Regression (MLR) with the performance of TB officers from 2014-2017. Data obtained by interviews and observations using questionnaires and analyzed by multiple regression α=0,05. The results of research are showed that statistically, both partially and simultaneously, from all of respondents have assessed according to several characteristics, namely age, sex, education, years of service and marital status that the distribution of respondents from each variable of compensation, supervision and competence are affected to the performance of TB officers at Puskesmas in the Working Area of the Medan City Health Service. The compensation variable is the most influential variable on the performance of Puskesmas TBC officers in the Working Area of Medan City Health Service.