The enactment of Law no 21/2019 has increased the effectiveness of preventive measures on the spread of Fish Quarantine listed Pests and Diseases (FQPD). Under new regulations, each FQPD host must pass laboratory tests to guarantee its FQPD-free status before translocated. This study was conducted to assess the risks of FQPD potentially contracting fishes translocated from Yogyakarta. Descriptive analysis was used by collaborating evaluation results of FQPD risk analyst team of FQIA Yogyakarta, standards and related regulations, and FQPD research results of various sources. The assessments listed 12 viral and 4 bacterial diseases having a high risk to spread. Status of high risk dominated assessment results with the level of risks varied from 18 to 25. Most diseases are the target for health certification and disease surveillance except TILV and RSIVD in Gouramy, VNN and E. ictalurii in Tilapias, CMNV in Vannamei, WSSV in Cherax, YHD in Tiger Prawn, and E. ictalurii in Walking Catfish as the sole target for surveillance; and VNN in Milk Fish as target only for certification. Assessment results are the basis for the determination of test targets of each FQPD host translocated from Yogyakarta.