The NOTCH gene encode transmembrane receptor. It play a vital role in several process stem cell maintenance and differentiation during embryonic and adult development. When ligand bind at a specific part intracellular part of NOTCH receptor is cleaved and translocate to the nucleus from where it can bind to transcription site. NOTCH activity can promotes tissue growth and cancer in some conditions but they also suppress tumors formation in others. Their gene structure show the amount of introns and exons by a dimensions structure of NOTCH gene. Various tools or database such as Mega7, Pfam and Gene structure and display server are used to analyze their phylogeny and their chromosome positions gene structure and introns and exons. Further studies are made to target the NOTCH pathway on growth and cancer suppressor.