DLX is a group of genes that is part of the homeobox-containing superfamily, which is involved in a variety of morphogenetic processes. Dlx genes occur as several paralogues in the invertebrate genome, resulting from tandem replication accompanied by whole-genome duplication. Despite the growing number of genome sequence tools in various vertebrates, embryological study, Dlx expression and function remains a challenge in terms of species diversity. Dlx gene family is in between the multigene families encoding for homeodomain-containing the transcription factors that are expressed earlier in the cells for the cranial neural crest and later in craniofacial mesenchyme and skeletal tissues. Gene structure analysis shows the number of exons and introns by a 3-dimensional structure of the Dlx gene. The Dlx gene family exons and introns are found to be very similar during this analysis. The distribution of genes across chromosomes was very varied. Collectively the newly discovered genes provide data for manipulating the Dlx genome to develop organs in vertebrates.