: Rue (Ruta angustifolia (L.) Pers.) Including rare medicinal plants. Cultured in vitro method is one alternative method of propagation in plants inggu. The study was conducted from July to December 2011 at the Laboratory of Botany, Nasional University Integrated Laboratory. The purpose of this study is to obtain the best BA concentration on shoot multiplication of rue. Explants used in the form of a stem segment (segment 1-10) of the rue plant. The medium used was MS medium with the addition of PGR ¾ BA in some degree of concentration as the treatment is 1.0, 2.0, 3.0; 4.0 and 5.0 ppm of 2,4-D was added to 0.3 ppm, and five replications. Data processing is done by using SPSS 19 for Windows. The results showed that administration of PGR combination of BA with different concentrations of 2,4-D +0.3 ppm in ¾ MS medium responded in the form of plant establishment and shoot multiplication rue. The analysis of variance showed that administration of BA with a concentration of BA 1.0, 2.0, 3.0; 4.0 and 5.0 ppm effect at both the number and length of shoots rue.