An analysis of methodologies and research papers on project portfolio management in various sectors of the econ-omy. The peculiarities of the subject branch (motor transport enterprises) are analyzed. The expediency of implementation of hybrid projects and development of tools for their management is substantiated. It is established that one of the unsolved management tasks is the task of forming effective portfolios of hybrid projects of motor transport enterprises. The purpose of the study is to substantiate the peculiarities of the formation portfolios of hybrid projects of trucking companies. And on their basis to describe the system relationships between operational, project and portfolio management, which provides quality tools for solving basic management problems of forming effective portfolios of hybrid projects of trucking com-panies. The scientific novelty of the performed researches is the substantiated features of the formation portfolios of hybrid projects of motor transport enterprises and the system interrelations between operational, project and portfolio management are described. As a result of the performed researches, the approach to the formation portfolios of hybrid projects of motor transport enterprises which are based on the account of the specificity of subject branch and system interrelations between operational, project and portfolio management is proved. This approach underlies the development of quality tools for the portfolio management of hybrid projects of trucking companies. It is established that based on the management of separate hybrid projects knowledge on their value and risk which together with the information on a condition of the design environment and features of portfolios of hybrid projects of the motor transport enterprises, is a basis of their qualitative formation is received. The unique characteristics of products (transport services) of individual projects, as well as actions for their creation and knowledge about them, are identified.