This research aimed to know the effect of using virtual laboratory PhET towards students' motivation in learning science on linear motion. This research is quasy experimental research that uses posttest only control group design. The population of this research is all grade VIII students of MTs Negeri 1 Ambon in the academic year 2020/2021 and the sample of this research is grade VIII4 as experimental group and VIII5 as control group that determined by simple random sampling technique. The instrument that used to collect the data is questionnaire of students' motivation in learning science which have 46 items (22 positive questions and 24 negative questions). The data The data were processed in the percentage and analysed by statistical formula of t-test by SPSS 26. The result of t-test shows that Sign. 0000 < 0,05, it mens that there is a significant difference effect of students' motivation in learning science on the linear motion. Therefore, it can be concluded that implementation of virtual laboratorial PhET has positive effect towards students' motivation in learning science on the linear motion.