The development of Autoplay Media Studio-based Temperature and Heat Teaching Materials aims to produce Autoplay media studio-based physics teaching materials to improve student learning outcomes by making the learning process easier and more interesting. This research was conducted at SMA Negeri 1 Tondano by taking temperature and heat material in class X IPA 2 with 28 students in the odd semester of the 2019-2020 school year. This study uses the Research and Development (R&D) method with four-D-models developed by Thiagarajan, Semmel and Semmel. This model is divided into 4 stages, namely Define, design, develop and disseminate. The teaching materials developed have passed the testing process by experts and were tested on students in small groups to obtain student responses and then tested in large groups or real classes and analyzed the learning outcomes. The results showed that the teaching materials developed had an average percentage of 88% of material expert validation results, 97% of media expert validation results and included in the very strong category. Student responses to teaching materials based on Autoplay Media Studio in the small group test gave a response of 96.3% which was included in the very strong category. The experimental method used is the design by subject method where the sample is given different treatment in two periods. In the first period students were taught using a conventional model using media commonly used in the classroom and in the second period students were taught using the Autoplay media studio developed in this study. As a prerequisite for normality testing, the significant value obtained in period 1 is 0.297 and period 2 is 0.256 which is greater than the significance level of 0.05, so it can be concluded that both data in period 1 and period 2 are normally distributed. The results of hypothesis testing are assisted by the application of SPSS 16.0 values |t hitung>t table| or |19,483>2.473| and based on a significance <0.05 then H0 is rejected and it is concluded that the average learning outcomes of students who are taught using physics teaching materials based on autoplay media studio are higher than the learning outcomes of students who are taught using conventional models.