The use of virtual laboratories of the Basic Electronics courses is very important as a solution to overcome the limited facilities in real laboratories at Sariputra Indonesia University, Tomohon. Virtualization of the Basic Electronics laboratory can function like a real laboratory that utilizes computer media and the Electronics Workbench (EWB) software in learning. The specific purpose of this study was to determine the effect of laboratory virtualization based on Electronics Workbench (EWB) on the learning outcomes of second semester students of the Informatics Engineering Study Program in Basic Electronics at Sariputra Indonesia University, Tomohon. The method used is the Pre-Experiment One-Group Pretest-Posttest Design with paired sample t-test analysis testing with the research sample taken by total sampling. The research hypothesis was analyzed with the SPSS application to obtain t count with a confidence level of 95% where t count > t table, that result 19,126 > 2,055 and based on the significance value of Sig. (2-tailed) 0.000 < 0.05, it can be concluded that there is a significant effect of based laboratory virtualization based on EWB to students learning outcomes of Informatics Engineering UNSRIT Semester II Basic Electronics material transistor amplifier. Furthermore, the N-Gain score test was carried out to see the effectiveness of using laboratory virtualization based on EWB to improving the learning outcomes of UNSRIT Informatics Engineering students with a result of 0.40 which was included in the moderate category. The output targeted in this study is expected to be published in a SINTA-accredited National journal 4 Journal of Study, Innovation and Physics Education Application p-ISSN: 2460-9587, i-SSN: 2614-7017 Study Program of Physics Education, Faculty of Teacher Training and Education, Muhamadiyah University, Mataram.