Ultraviolet (UV) is one of the lights spectrum that causes plant cell to damage. The use of ultraviolet irradiation in combination with tissue culture techniques has not been widely used in somaclonal variation method for carnation breeding, whereas the source of ultraviolet irradiation is more easily obtained compared to other sources of irradiation such as gamma rays, x-rays and neutron rays. The purpose of this study was to determine effect of ultraviolet irradiation on the multiplication of axillary shoot in carnation. Explants of internode stem of carnation were grown in multiplication medium Murashige Skoog (MS) consisted of 10-7 M NAA and 5.10-6 M BAP. After four times subculture, the culture bottles were exposed to ultraviolet light C with some variation of irradiation time in combination with storage in light or dark. The results showed that ultraviolet light can reduce the number of shoots formed, shoot length and chlorophyll content of carnation. Based on the results obtained, ultraviolet radiation affected the organs of plants that were formed, but did not cause meristem activity to a standstil.