AbstactCaharcterization of Cucumis sativus germplasms was done to get the description of plant character for the breeder to use as &ldquo;Working Collection&rdquo; materials which were useful in breeding programme. Characterization was done on 23 accessions of Cucumis sativus germplasm planted at Subang station (100 m asl) during dry season 2003. Twenty seeds of each accession planted in a plot with planting distance of 70 x 40 cm. The description of Cucumis sativus germplasms had been done. Six accessions of Cucumis sativus were selected for Working Collection based on fruits quality and resistance to environmental stresser.