Hybridization program has been used to increase fish production for more than three decades. Concern to this program is due to the problem of potential impact on wild population. Hence, it is urgently needed to provide quick identification tools in the field and as well as commercial aspect in term of growth rate. This study investigated biometric characters, growth performance, and ovarian development of Pangasius djambal and Pangasianodon hypophthalmus and their reciprocal hybrids. A detailed morphological analysis was done on the hybrids of P. djambal and P. hypophthalmus. Earthen pond on the growth comparison as well as the ovarian development was also observed. The results of morphometric and meristic showed that the reciprocal hybrids have intermediate characters except for gill rakers numbers in which lower than that of parental species. Growth analysis showed that hybrids has better performance compare to the parental one, while ovarian development of hybrids was developed abnormal. No oocytes reached vitellogenesis and showed major atresia and fatty. It can be concluded that hybrids of P. djambal and P. hypophthalmus is true hybrids. The hybrids can be used as final seed for aquaculture production due to clearly identify from parents, superior growth performance and steril.