. Anopheline species confirmed as malaria vector if the salivary gland contained sporozoites. One of the method to confirmed it was through an Enzyme-Linked Immunosorbent Assay (ELISA). The aim of this study was to investigate the presence of circum sporozoite protein (CSP) in the mosquito of Anopheles barbirostris with ELISA method. The study was conducted in malaria endemic area named Modu Waimaringu Village, Waikabubak District, Sumba Barat Regency in March 2011. The study design was cross-sectional study, mosquito for the ELISA test were collected only from animal bait. ELISA method examination used on An. barbirostris body parts (i.e. the head-thorax) where sporozoites of P. falciparum or P. Vivax possibly be found. The results showed that 40 samples of An. barbirostris mosquitoes which acquired from the mosquite bait in Modu Waimaringu Village was negative (100%). It means that there was no CSP found and An. barbirostris was not a malaria vector in the area.