Star fruit (Averrhoa carambola) is one of tropical fruits which had a high content of vitamin C, which was higher than that in apple and grape. As fresh consumption, star fruit had a good role in decreasing human blood pressure. Main constraints of star fruit development weather for conservation purpose and or for cultivation were still limited due to lack of seedlings availability. In vitro culture technique was one of the altrnative technologies capable of producing seedlings in a large quantity, uniform growth and relatively in a short period. One of the important keys in micropropagation work was the step of shoot initiation and multiplication. In this study we used two kind of explant, namely shoot with single node and shoot from germinated embrio. Experiment I, shoot with single node and shoot from germinated embrio were planted at WPM media + citric acid 100 mg/l. The next activities was focused on single node shoots which was subcultured at WPM + BAP 0.5 mg/l. In experiment II in vitro shoots from previous experiment was subcultured at WPM + BA (1 and 2 mg/l) + thidiazuron (0.1 and 0.2 mg/l). To stimulate shoot multiplication rate, shoot was subcultured at WPM or MS media in combination with IAA 0.5 mg/l and zeatin 2 mg/l. To improve vigourity of the plant, in vitro shoots resulted from multiplication media was planted at WPM or MS media containing paclobutrazol (0.4 and 0.8 mg/l) + BA 2 mg/l + thidiazuron 0.2 mg/l. Result showed that the use of single node shoot as an explant better than shoot comes from germinated embrio. Sub culture of star fruit shoot on WPM basal media containing BAP of 0.5 mg/l produce the shoot number about 4, and the shoot number could be increased until 18 by using IAA 0.5 mg and zeatin 2 mg/l. The treatment of schock temperature at 4-5oC during 4 days before planting could fasten shoot initiation time from 3 months to 1 months. An addition of 0.4 mg/l paclobutrazol on MS or WPM media containing 2 mg/l BA and 0.2 mg/l thidiazuron could improve vigourity of plantlet.