. Karuni village is located in work area of Radamata clinic in West North Sumba regency, which has AMI of 155,37‰ in 2010, and 86,91‰ in 2011. The diversity of Anopheles spp. determines the malaria incidence and control; therefore bioecology research of Anopheles spp. was conducted. Data was collected by cross sectional method, the population used is the whole Anopheles spp. that exists in Karuni village and the sample is Anopheles spp. that were caught and observed in breeding habitat. Adult mosquitoes data collection was conducted by human bait collection and resting method, pre adult mosquitoes collection captured in breeding habitat such as buffalo wallow, rice field, gutter, swamp and ex pond. The species found were An. vagus, An. aconitus and An. barbirostris. Based on night catching collection, 5 species were found, those are An. aconitus, An. tesselatus, An. annularis, An. vagus and An. barbirostris. An. aconitus has the highest Man Hour Density compared to the other four species (MHD=0.67 man/hour) at 10-11 pm, Anopheles vagus has the second highest Man Hour Density (MHD=0.58 man/hour) at 9-10 pm. The average of Anopheles MHD is 0.02-0.17, most of the mosquitoes found from inside the house collection and cattle cage collection. In conclusion, the breeding places that were found in this research are a suitable habitat for breeding Anopheles larvae. The behavior of Anopheles spp. tends to be endofagic and exofilic. Control efforts of Anopeheles spp. is carried out by chemical controlling, biologic and physical environmental controlling.