One of the effort for controlling Aedes aegypti as dengue vector is using synthetic larvacide such as temephos. Continuous and repeating utilization of temephos may increase risk of resistance. The objective of this study was to determine the susceptibility of Ae. aegypti larvae against temephos in endemic areas of dengue fever in Tasikmalaya. This was experimental study with a block randomized design. Population of larvae Ae. aegypti were in dengue fever endemic area houses from Tasikmalaya. A total of 700 larvae of Ae. aegypti, were obtained from houses in each endemic area and have been bred to third descendants (F3). Susceptibility temephos was tested by Elliot and Polson method used the WHO diagnostic dose 0.02 ppm. Results showed that the LC95 of temephos were 0.00926 ppm in Kelurahan of Sukamanah, 0,01015 ppm in Kelurahan of Cikalang, 0.01137 ppm in Kelurahan of Kersangara, and 0.02045 ppm in Kelurahan of Tugujaya was indicated resistant to tempehos.