Three anoxygenic photosynthetic bacteria, DS-1, DS-4 and Cas-13, have been examinated for themorphological and physiological properties. All strains were rod-shape cells with a swollen terminal endGram negative, motile, non-halophilic, non-alkalophilic and non-acidophilic, and capable of utilizinbenzoate aerobically and photo-anaerobically. Sequence analysis of part of 16S rRNA genes showed that DS1 and Cas-13 were closely related to Rhodopseudomonas palustris Strain 7 with a similarity of 97%, whereaDS-4 may not be closely related to the former two strains with a similarity of 78% based on the constructephylogenic tree. Spectral analysis indicated that the three bacteria had bacteriochlorophyl a and normaspirilloxanthin series. Growth in medium enriched with vitamin and supplemented with benzoate as their sole C-sources wabetter than in medium without vitamin. Benzoate degradation in medium with vitamin was accelerated. Thability to grow on benzoate without added vitamins indicated that the bacteria were able to synthesize theown vitamins.